Safety Assays
In vitro safety testing can be used to identify adverse outcome pathways (AOP), targets of toxicity critical to understanding overall chemical hazard and for performing risk assessment.
We validate the safety of your deodorizing, sanitizing and disinfectant products, through New Approach Methodology / non-animal alternative methods (NAMs), per standards from OECD. Learn about our tests:
In vitro skin irritation
Evaluation of the potential for skin irritation in vitro after topical application of the investigational product in a three-dimensional model of RhE, composed of untransformed epidermal keratinocytes of human origin, and cell viability was evaluated by MTT.
OECD TG 439 – In Vitro Skin Irritation: Reconstructed Human Epidermis Test Methods.
Acute eye irritation
OECD TG 437 – Bovine Corneal Permeability and Opacity Test: Evaluation of irritation in rabbit corneal cell culture (SIRC) with two concentrations of the test substance (5% and 0.05%). After a brief exposure time a cell viability assay (MTT Test) is performed to measure the viability of the cells.
OECD 492 – Reconstructed human Cornea-like Epithelium (RhCE) test method for identifying chemicals not requiring classification and labelling for eye irritation or serious eye damage: The test chemical is applied topically to a minimum of two three-dimensional Reconstructed human Cornea-like Epithelium (RhCE) tissue constructs and tissue viability is measured following exposure and a post-treatment incubation period. RhCE tissue viability is classically measured by enzymatic conversion of the vital dye MTT by the viable cells of the tissue into a blue MTT formazan salt that is quantitatively measured after extraction from tissues. Test System: SkinEthicTM HCE / Human Corneal Epithelium.
Acute oral toxicity
The method provides information on the hazardous properties and allows the substance to be classified for acute toxicity according to the Globally Harmonised System of classification and labelling of chemicals.
OECD TG 129 – Estimation of starting dose for acute oral systemic toxicity testing.
Mutagenicity
The chemical is exposed to test in the presence and absence of metabolic activation, either by plate incorporation or by pre-incubation prior to plating out. Mutations are determined by scoring bacterial growth (revertant colonies) on selective agar plates lacking the essential amino acid. Five different analysable concentrations of the test substance will be used. Recommended combination of strains is:
- S. typhimurium TA1535
- S.typhimurium TA1537
- S. typhimurium TA98
- S. typhimurium TA100
- S. typhimurium TA102
- E. coli WP2 uvrA (pKM101)r.
OECD 471 – GUIDELINE FOR TESTING OF CHEMICALS Bacterial Reverse Mutation Test